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GENETICA INVERSA BASE PLASMIDOS [1 record]
Record 1 - internal organization data 2006-11-28
Record 1, English
Record 1, Subject field(s)
- Biotechnology
- Genetics
- Immunology
Record 1, Main entry term, English
- plasmid-based reverse genetics
1, record 1, English, plasmid%2Dbased%20reverse%20genetics
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
Plasmid-based reverse genetics lets scientists custom-manufacture flu viruses. DNA [deoxyribonucleic acid] copies of genes from two different flu strains are inserted into DNA rings called plasmids. The gene-bearing plasmids are then injected into a culture of living cells, which manufacture whole flu viruses containing the desired combination of genes. 2, record 1, English, - plasmid%2Dbased%20reverse%20genetics
Record 1, French
Record 1, Domaine(s)
- Biotechnologie
- Génétique
- Immunologie
Record 1, Main entry term, French
- génétique inverse à base de plasmides
1, record 1, French, g%C3%A9n%C3%A9tique%20inverse%20%C3%A0%20base%20de%20plasmides
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
La génétique inverse à base de plasmides permet la fabrication de virus de la grippe. Des copies d'ADN [acide désoxyribonucléique] de gènes provenant de deux souches de grippe différentes sont insérées dans des anneaux d'ADN nommés plasmides. Ces plasmides porteurs de gènes sont introduits dans une culture de cellules qui fabriquent des virus de grippe complets contenant la combinaison de gènes souhaitée. 1, record 1, French, - g%C3%A9n%C3%A9tique%20inverse%20%C3%A0%20base%20de%20plasmides
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Biotecnología
- Genética
- Inmunología
Record 1, Main entry term, Spanish
- genética inversa a base de plásmidos
1, record 1, Spanish, gen%C3%A9tica%20inversa%20a%20base%20de%20pl%C3%A1smidos
proposal, feminine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
- clonación posicional a base de plásmidos 1, record 1, Spanish, clonaci%C3%B3n%20posicional%20a%20base%20de%20pl%C3%A1smidos
proposal, feminine noun
Record 1, Textual support, Spanish
Record number: 1, Textual support number: 1 OBS
La principal dificultad a la que se enfrentan los científicos es que el virus de la gripe aviar que circula por Asia y parte de Europa oriental es tan virulento que no puede multiplicarse en huevos. Su patogenicidad es tan elevada que los embriones de aves mueren por su infección. El equipo del profesor Kawaoka ha podido sortear este obstáculo con la tecnología de la genética inversa, que permite ensamblar los dos genes principales del virus patógeno, convenientemente desactivados, con seis genes de un inocuo virus gripal en pequeños fragmentos de ADN llamados plásmidos, que posteriormente son insertados en células renales de mono. 2, record 1, Spanish, - gen%C3%A9tica%20inversa%20a%20base%20de%20pl%C3%A1smidos
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