TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

Pyrosequencing is a method of DNA sequencing that differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release and the generation of light on nucleotide incorporation, rather than chain termination with dideoxynucleotides.  2, record 1, English, - pyrosequencing

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 CONT

Le pyroséquençage est une technique de séquençage de l'ADN qui permet d’effectuer un séquençage rapide et à moindre coût qu'un séquençage par la méthode de Sanger. En effet, cette technique ne nécessite pas de clonage(donc gain de temps et d’argent), et permet une lecture directe de la séquence obtenue après le séquençage.  2, record 1, French, - pyros%C3%A9quen%C3%A7age

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

Two decades of culture-independent studies have confirmed that microbial communities represent the most complex and concentrated pool of phylogenetic diversity on the planet. There remains a need for innovative molecular tools that can further our knowledge of microbial diversity and its functional implications. We present the method and application of serial analysis of ribosomal sequence tags (SARST) as a novel tool for elucidating complex microbial communities, such as those found in soils and sediments. Serial analysis of ribosomal sequence tags uses a series of enzymatic reactions to amplify and ligate ribosomal sequence tags (RSTs) from bacterial small subunit rRNA gene (SSU rDNA) V1-regions into concatemers that are cloned and sequenced.  2, record 2, English, - serial%20analysis%20of%20ribosomal%20sequence%20tags

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

Les techniques faisant partie du séquençage à haut débit comprennent l'analyse sériée d’étiquettes de séquences ribosomiques(SARST) et les plateformes de séquençage de la prochaine génération comme le pyroséquençage 454, l'Illumina et le séquençage par ligation(SOLiD). Ces méthodes génèrent des milliers de séquences, permettant une grosse couverture de la diversité bactérienne.  3, record 2, French, - analyse%20s%C3%A9ri%C3%A9e%20d%26rsquo%3B%C3%A9tiquettes%20de%20s%C3%A9quences%20ribosomiques

Record 2,  Textual support,  Spanish