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PEIGNAGE MOLECULAIRE [1 record]
Record 1 - internal organization data 2010-04-20
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- molecular combing
1, record 1, English, molecular%20combing
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
In a process called "molecular combing", DNA molecules attached at one end to a solid surface were extended and aligned by a receding air-water interface and left to dry on the surface. Molecular combing was observed to extend the length of the bacteriophage Lambda DNA molecule to 21.5+/-0.5 micrometers (unextended length, 16.2 micrometers). 1, record 1, English, - molecular%20combing
Record number: 1, Textual support number: 1 OBS
With the combing process, it was possible to: (i) extend a chromosomal Escherichia coli DNA fragment (10^6 base pairs) and (ii) detect a minute quantity of DNA (10^3 molecules). These results open the way for a faster physical mapping of the genome and for the detection of small quantities of target DNA from a population of molecules. 1, record 1, English, - molecular%20combing
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- peignage moléculaire
1, record 1, French, peignage%20mol%C3%A9culaire
correct, masculine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- peignage moléculaire d'ADN 2, record 1, French, peignage%20mol%C3%A9culaire%20d%27ADN
correct, masculine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
Le peignage moléculaire de l'ADN, découvert en 1994, repose sur deux principes simples : l'ancrage spécifique, dans des conditions physico-chimiques précises, de molécules individuelles d'ADN par leurs extrémités sur des surfaces traitées ou non; la force de traction développée par un ménisque en mouvement; c'est-à-dire la force exercée sur tout objet placée sur la ligne de contact d'une solution avec une surface, lors du déplacement de cette ligne. 3, record 1, French, - peignage%20mol%C3%A9culaire
Record number: 1, Textual support number: 2 CONT
[...] la technique de peignage moléculaire, destinée à étaler des macromolécules (notamment l'ADN), parallèlement les unes aux autres, en les ancrant spécifiquement par leurs extrémités [...]. 4, record 1, French, - peignage%20mol%C3%A9culaire
Record number: 1, Textual support number: 1 OBS
Les applications du peignage moléculaire sont la cartographie génétique, le diagnostic génétique et la PCR in situ. 5, record 1, French, - peignage%20mol%C3%A9culaire
Record 1, Spanish
Record 1, Textual support, Spanish
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