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SLS [1 record]
Record 1 - internal organization data 2012-09-19
Record 1, English
Record 1, Subject field(s)
- Biological Sciences
Record 1, Main entry term, English
- sodium dodecyl sulphate
1, record 1, English, sodium%20dodecyl%20sulphate
correct
Record 1, Abbreviations, English
- SDS 2, record 1, English, SDS
correct
Record 1, Synonyms, English
- sodium lauryl sulfate 3, record 1, English, sodium%20lauryl%20sulfate
correct
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
Ionic detergent used for denaturation of protein and in electrophoresis of protein subunits. 1, record 1, English, - sodium%20dodecyl%20sulphate
Record number: 1, Textual support number: 1 CONT
Detergent solubilization has been used with great success to study the major proteins in various membranes. Typically, the membranes are first heated to 100°C in a 1% solution of the strong detergent SDS (which disrupts all non-covalent protein-protein and protein-lipid interactions and completely unfolds the proteins), then layered on top of a polyacrylamide gel containing SDS, and lastly subjected to electrophoresis in a strong electric field for several hours. The amount of anionic SDS bound to protein is roughly equal to the mass of the polypeptide, with the result that the negative charge of the detergent overwhelms the intrinsic charge of the protein. Consequently, each individual protein migrates in the electric field at a rate determined by its molecular weight. 4, record 1, English, - sodium%20dodecyl%20sulphate
Record 1, French
Record 1, Domaine(s)
- Sciences biologiques
Record 1, Main entry term, French
- dodécyl sulfate de sodium
1, record 1, French, dod%C3%A9cyl%20sulfate%20de%20sodium
correct, masculine noun
Record 1, Abbreviations, French
- SDS 2, record 1, French, SDS
correct
Record 1, Synonyms, French
- laurylsulfate de sodium 3, record 1, French, laurylsulfate%20de%20sodium
correct, masculine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Détergent ionique employé comme agent dénaturant des protéines. 2, record 1, French, - dod%C3%A9cyl%20sulfate%20de%20sodium
Record number: 1, Textual support number: 1 CONT
Lors de l'électrophorèse en gel filtrant les protéines sont séparées selon leur taille moléculaire (ou encombrement stérique) et leur charge électrique. L'encombrement stérique d'une molécule dépend du poids moléculaire et de la configuration spatiale de la protéine. Pour mesurer le poids moléculaire d'une protéine, il faut donc éliminer l'influence de la charge et de la configuration spatiale. Un agent dénaturant, le dodécyl sulfate de sodium, permet d'obtenir ce résultat. Grâce à son affinité pour les protéines sur lesquelles il se fixe très solidement et en grande quantité, il leur confère une charge électrique constante par unité de surface et modifie considérablement leur configuration spatiale. C'est pourquoi le traitement par le SDS permet de mesurer directement le poids moléculaire d'une protéine après électrophorèse dans un gel de polyacrylamide. 4, record 1, French, - dod%C3%A9cyl%20sulfate%20de%20sodium
Record number: 1, Textual support number: 1 OBS
SDS est une abréviation de dodécyl sulfate de sodium. 2, record 1, French, - dod%C3%A9cyl%20sulfate%20de%20sodium
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Ciencias biológicas
Record 1, Main entry term, Spanish
- dodecil sulfato sódico
1, record 1, Spanish, dodecil%20sulfato%20s%C3%B3dico
correct, masculine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
- laurilsulfato sódico 2, record 1, Spanish, laurilsulfato%20s%C3%B3dico
correct, masculine noun
Record 1, Textual support, Spanish
Record number: 1, Textual support number: 1 DEF
Detergente utilizado para solubilizar proteínas y ADN de muestras biológicas. 1, record 1, Spanish, - dodecil%20sulfato%20s%C3%B3dico
Record number: 1, Textual support number: 1 OBS
Sólido de color blanco o amarillo. Soluble en agua. Poco tóxico. 2, record 1, Spanish, - dodecil%20sulfato%20s%C3%B3dico
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